Cinder Biological, Inc.

Cinder Biological, Inc., operating as CinderBio, is a biotechnology research company that develops and manufactures advanced hyperthermoacidic archaeal (HTA) proteases. These enzymes are derived from microbes that thrive in extreme hot and acidic environments. By leveraging these unique biological properties, the company has created a suite of products, such as its Krakatoa and Vesuvius proteases, which function efficiently at high temperatures and low pH levels. These HTA-proteases allow for rapid, single-step protein digestion that replaces traditional, slower, and more complex workflows, such as those relying on trypsin. Their technology enables enhanced proteome coverage and provides orthogonal datasets, making them particularly effective for challenging samples including membrane proteins and nucleoproteins.

These innovative enzymes are used in proteomics, clinical research, and various industrial applications where process efficiency and stability are critical. By eliminating the need for chaotropes and simplifying sample preparation into a rapid process—often under one hour—CinderBio's solutions provide significant time and cost advantages to scientists and industrial partners. The company’s technology has been validated through peer-reviewed research, demonstrating increased unique peptide reads and improved de novo antibody sequencing capabilities. Beyond laboratory research, the company has explored industrial applications, including enzymatic cleaning and sanitation products, and processes for the liberation of sugars from cellulosic materials. Based in San Leandro, California, CinderBio continues to collaborate with the scientific community to integrate its extreme-environment enzymes into novel workflows.