BioMosae evolved a streamlined approach for the discovery and identification of novel AI’s by chaining multidisciplinary and complementary methodologies. The systematic discovery approach comprises multiple sequential and parallel stages: • In silico data mining of available genomic DNA for initial prediction of potential AI • Overproduction of AI by liquid cultivation and coupled growth optimization of target organisms • Preparative isolation and enrichment of AI • Correlation of purified isolates to antifungal efficacy by in vitro/in vivo growth inhibition assays • Structural identification of novel AI by instrumental analytics and proteomics • Relative and absolute quantification of AI • Description, identification and quantification of background matrix compounds.