Isoform-specific screening assay for 5-alpha reductase inhibitors using dual-substrate mass spectrometry
A cell-based high-throughput screening platform that distinguishes inhibition of steroid 5-alpha reductase isoforms 1 and 2 in a single assay. By co-expressing SRD5A1 and SRD5A2 in HEK293 cells and using isoform-selectiv...