Automated colony-to-array screening for recombinant protein discovery
Automated front end for recombinant protein discovery using microbial or algal expression systems. PIXL Max images, detects and selects colonies by morphology, size, colour or fluorescence, then isolates chosen clones into 96- or 384-well plates. ROTOR+ creates reproducible arrays and replicates them across alternative media, induction conditions or phenotype assays, enabling parallel comparison of large clone libraries with matched plates for downstream protein production, quality control and functional screening.
Overview
Singer Instruments offers an automated front end for recombinant protein discovery using microbial or algal expression systems. The solution combines PIXL Max, a colony imaging and picking instrument, with ROTOR+, a high-density arraying and replication instrument, enabling parallel comparison of large clone libraries while creating matched plates for downstream protein production, quality control and functional screening.
The workflow reduces repetitive colony handling and plate replication while allowing protocols to change between early-stage experiments. Data outputs and instrument control can be connected to existing automation, LIMS and downstream assays through an open API.
Technical specifications
Key capabilities:
- PIXL Max detects colonies down to 0.2 mm, picks up to 3,000 colonies per hour and provides greater than 99 percent transfer efficiency
- Colony selection based on morphology, size, colour or fluorescence
- Isolates chosen clones into 96- or 384-well plates
- ROTOR+ replicates an array in under 25 seconds and can screen at close to one million colonies per hour
- Array densities from 96 to 6,144 colonies
- Replication across alternative media, induction conditions or phenotype assays
- Sterile, disposable PickupLine tips and agar-surface detection protect sample integrity
- Images, colony metadata and experimental logs support traceability
- Demonstrated use across bacteria, fungi and algae
Technology readiness level
Both systems are available at technology readiness level 9 and are in commercial use. Validation is proposed through a proof-of-concept programme using representative constructs and conditions, benchmarking throughput, hands-on time, recovery and reproducibility before integration and scale-up. Custom labware, workflows and software integration can be co-developed as programmes evolve.
About Singer Instrument Co LTD
Singer Instruments is a long-standing developer and manufacturer of laboratory automation equipment, specializing in mechatronic workstations and robotics that support genetic and genomic research. Founded in 1934, the company designs, programs, and assembles its core products at its state-of-the-art facility in Exmoor National Park, Somerset, England. Their portfolio includes flagship automation solutions such as colony pickers, which are widely used by researchers to accelerate biological workflows in fields ranging from microbiology and neuroscience to cancer biology and biofuel engineering. By emphasizing high-quality precision engineering and in-house manufacturing, the company maintains rigorous quality control while providing specialized technical support to a global scientific community.
The company serves public and private research institutions across more than 60 countries, aiming to accelerate scientific discovery through dependable automation. Singer Instruments distinguishes itself through deep collaboration with the genetics community and a commitment to customer-driven innovation, which has facilitated the development of tools integrated into numerous high-impact scientific publications. Their global presence, supported by offices in North America and Europe, enables them to maintain a strong partnership with laboratory professionals, helping to solve complex procedural challenges while ensuring long-term product reliability in demanding research environments.
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