Culture-free molecular diagnostic method for bloodstream infection based on detection of neutrophil phagocytes in blood
We propose an innovative approach to diagnosis of bloodstream infections based on detection of neutrophil phagocytes circulating in the bloodstream. We have developed droplet digital cell PCR that enables detection of bacterial DNA in neutrophils at the single cell level. The method eliminates both cell lysis and nucleic acid extraction but allows us to amplify pathogen DNA intracellularly and detect amplicons with fluorescence. We would like to expand the method into many pathogens and make it implementable for clinical use. The method is described in detail in the article published recently in Communications Materials. We would love to hear from you, if you are willing to support our project and improve diagnosis of bloodstream infection together.
About Nagasaki University
Nagasaki University is a large, comprehensive national research university in Nagasaki City, with more than 9,000 students and an international, practice-oriented character. Its university hospital, the only university hospital in Nagasaki Prefecture, links research with advanced care and clinical study, while the Institute of Tropical Medicine operates shared facilities, joint-use programs, and overseas stations in Kenya and Vietnam. An open-innovation platform and joint research office connect university capabilities with company needs, support commissioned and collaborative research, and help move results toward commercialization. Research draws on MEXT and JSPS Grants-in-Aid, AMED, JST, and JICA-linked national programs. Research promotion staff support external funding, intellectual property, and partnership development.
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