Accelerating flowering in canola via gene and promoter editing

Technology
In development
University

Vineland Research and Innovation Centre offers a contracted service to accelerate canola flowering through targeted gene and promoter editing using CRISPR. This solution leverages flowering-time regulators from Arabidopsis to generate allelic series, enhancing commercial product development.

Overview

The Vineland Research and Innovation Centre presents a cutting-edge service designed to accelerate flowering in Brassica napus (canola) by leveraging gene and promoter editing technologies. Utilizing well-characterized flowering-time regulators from Arabidopsis thaliana as a basis, this service employs CRISPR-based approaches to create a gradient of alleles. This innovative method accelerates flowering while maintaining yield stability, offering a strategic advantage in commercial product development.

Technical specifications
  • Gene Editing Approach: Utilizes CRISPR to target key flowering-time regulators in the A and C subgenomes of Brassica napus.
  • Allelic Series Creation: Designs guide RNAs for simultaneous targeting, enabling the creation of full knockouts and attenuated promoter variants.
  • Validation Process: Edited lines are evaluated under controlled conditions for flowering time, expression profiles, and agronomic traits.
  • Promoter Editing: Partial function is maintained through promoter editing to balance early flowering with yield stability.
Technology readiness level

This technology is currently at TRL 4, representing validation in a laboratory environment. Ongoing research aims to transition these findings to greenhouse trials and eventual integration into elite germplasm for commercial deployment.


About Vineland Research and Innovation Centre

Vineland Research and Innovation Centre is an independent, not-for-profit horticultural research organization in Vineland Station, Ontario, with a team of 100+ people focused on translating science into market-ready solutions. Its integrated campus combines pre-commercial greenhouses, advanced pathology and soil/substrate labs, a prototyping hangar for robotics and sensors, dedicated sensory and food technology suites, and field-scale research farms. A research-park setting with co-located government, academic and industry tenants enables on-site collaboration, testing and demonstrations. Vineland is funded in part through the Sustainable Canadian Agricultural Partnership, with support from Agriculture and Agri-Food Canada and the Government of Ontario. Commercialization is supported via defined licensing pathways and an active intellectual property portfolio.

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