Antagonist design targeting allatostatin C protein for rice stem borer pest control
Computationally designed small-molecule antagonists targeting the Allatostatin C neuropeptide pathway in Chilo suppressalis (Asiatic rice borer). Lead compounds have been identified through molecular docking and in-silico safety screening, offering a novel approach to disrupt insect physiology and protect rice crops.
Overview
This research presents a rational, structure-based approach to designing small-molecule antagonists that disrupt the Allatostatin C neuropeptide signaling pathway in Chilo suppressalis, the Asiatic rice stem borer. Neuropeptides and their G protein-coupled receptors (GPCRs) regulate critical insect physiological processes including development, reproduction, feeding, metabolism, and neuromuscular function. By targeting the Allatostatin C protein and its interaction with the A6-B protein of the thyrotropin-releasing hormone pathway, this work aims to interfere with metabolic rate, heat generation, neuromuscular function, and heart rate in the pest. The approach offers a promising new avenue for pharmacological insect pest management that could complement or reduce reliance on conventional insecticides.
Technical specifications
Key features:
- Transcriptomic identification of neuropeptide and GPCR targets specific to Chilo suppressalis
- Molecular docking-based screening to shortlist potential lead compounds against the Allatostatin C protein
- Proprietary safety profile screening to evaluate potential side effects of lead molecules upon consumption
- Targeting of the Allatostatin C–A6-B protein interaction within the thyrotropin-releasing hormone pathway, which acts as a master regulator of thyroid hormone signaling
- Disruption of downstream physiological processes including metabolic rate regulation, heat generation, neuromuscular function, and heart rate
- Planned force-feeding bioassays on lab-adapted C. suppressalis larvae at multiple developmental stages
- Mortality assessment at 24, 48, and 72 hours post-treatment with LD50 calculation per larval weight
- Thyroid level measurements to evaluate neuromodulation effects
Technology readiness level
The research is at an early to mid-stage of development. Lead compounds have been identified through in-silico molecular docking and passed initial computational safety screening. The next phase involves in-vivo validation through force-feeding bioassays on laboratory-adapted C. suppressalis larvae, with mortality quantification and LD50 determination. Future work will include affinity measurements to confirm the molecular interaction between Allatostatin C and the lead compounds, contingent on funding availability. The technology has not yet been validated in field conditions.
About R.V. College of Engineering
RV College of Engineering (RVCE) is an autonomous, self‑financing engineering institution in Bengaluru, affiliated to Visvesvaraya Technological University and accredited NAAC A+. It connects to industry through an active Industry Institute Interaction Cell, 150+ MoUs, and co‑located, industry‑sponsored labs and Centers of Excellence that enable joint training, prototyping, and upskilling on campus. Proximity to Bengaluru’s technology cluster supports internships, capstone co‑supervision, and consultancy engagements throughout the year. Research here is supported by competitive national programs and industry, including DRDO/NRB, AICTE, and ISRO collaborations. An IP Coordination Cell, together with incubation resources and a student‑run Entrepreneurship Development Cell, assists with patenting, licensing, and venture formation.
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