Anthocyanin-rich tomatoes developed via crispr/cas9 gene editing
This research utilizes CRISPR/Cas9 technology to enhance anthocyanin production in tomatoes by editing the SlMYB-ATV gene, achieving light-independent synthesis. The approach aims to produce transgene-free, anthocyanin-rich tomatoes efficiently.
Overview
This innovative approach leverages CRISPR/Cas9 gene editing to significantly enhance anthocyanin production in tomatoes by targeting the SlMYB-ATV gene. Anthocyanins are beneficial plant pigments with antioxidant properties, and their increased presence in tomatoes could offer enhanced nutritional benefits. Unlike traditional breeding or GM methods, this technique enables light-independent synthesis of anthocyanins throughout the tomato fruit, promising a more efficient and commercially viable pathway to produce health-enhanced tomatoes.
Technical specifications
The core of this technology lies in the precise editing of the SlMYB-ATV gene, a known repressor of anthocyanin biosynthesis. By knocking out this gene, the tomatoes can produce anthocyanins independent of light, overcoming limitations of current anthocyanin-rich varieties. Two strategies are being pursued: a full non-GM approach using Cas9-gRNA RNPs directly in protoplasts, and a mixed GM/non-GM strategy that initially uses GM lines to achieve editing before removing transgenes. Both methods aim to produce transgene-free, anthocyanin-rich tomatoes.
Technology readiness level
The technology is currently at TRL 2, indicating that the concept has been formulated and initial proof of concept has been achieved. Ongoing work includes the regeneration of edited plants and characterization of mutant lines, moving towards higher TRLs through further validation and development.
About The University of Queensland
The University of Queensland is a large, multi-campus public research university in Brisbane with a comprehensive academic and research profile. Industry partners engage through hospital-embedded laboratories and clinical sites, pilot testbeds, and field campuses across Queensland that enable prototyping, trials, and validation in real-world settings. Co-location with major health precincts and proximity to Brisbane’s innovation districts make collaboration and talent access straightforward, while on-campus incubation and professional education support workforce upskilling. Research is supported by competitive national funding from the Australian Research Council and the National Health and Medical Research Council, alongside state and industry investment. A dedicated technology transfer office and commercialization company manage IP, licensing, sponsored research agreements, and spin-out formation.
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