Antibiotic-free protein expression system with endotoxin-free product
Terra Bioforge offers a cutting-edge protein expression system using Bacillus subtilis and ClearColi E. coli strains for antibiotic-free and endotoxin-free recombinant protein production, enhancing yield and stability.
Overview
Terra Bioforge has developed an innovative protein expression system that eliminates the need for antibiotics, reducing endotoxin contamination in recombinant protein production. By employing genome-integrated gene expression in Bacillus subtilis and ClearColi Escherichia coli strains, this technology offers a stable platform for producing proteins without the traditional antibiotic markers. The approach not only addresses contamination concerns but also enhances the long-term stability of the production strains, making it ideal for various biotechnological applications.
Technical specifications
- Utilizes Bacillus subtilis and ClearColi E. coli strains for gene integration
- Genome integration without antibiotics, followed by precise deletion of markers
- Stability maintained for thousands of generations
- Optimization includes strong inducible promoters (e.g., T7, lacI), mRNA stabilizing sequences, and fusion tags (e.g., MBP, SUMO)
- Incorporates chaperone co-expression and other enhancements for increased solubility and expression
- Iterative optimization of gene dosage and fermentation conditions
Technology readiness level
This technology is at Technology Readiness Level 4, with established proof of concept and early validation in laboratory settings. Future development will focus on optimizing expression parameters to meet specific customer requirements.
About Terra Bioforge
Terra Bioforge is a synthetic biology company that specializes in microbial strain engineering for the discovery and production of valuable natural products. The company utilizes a proprietary platform that integrates genomics, metabolomics, AI-driven metabolite analysis, and data science to reprogram biosynthetic pathways. Their core technologies include MycoDrive, a genome engineering platform for filamentous fungi, and a Metabologenomics approach that maps microbial molecules to their specific genetic recipes. By offering custom genetic engineering services for bacterial and fungal strains—such as Bacillus, Paenibacillus, and Streptomyces—the company enables the creation of efficient microbial factories for various industrial applications.
These capabilities are designed to help pharmaceutical and agtech partners shorten R&D timelines, increase fermentation efficiency, and reduce production costs for complex chemistries. The company supports projects across human health, animal health, and agriculture, including tasks such as optimizing nutrient utilization, stable fluorescent labeling, and the secretion of bioactive peptides or enzymes. By enabling the scalable production of natural compounds, Terra Bioforge assists customers in bringing products to market faster and more cost-effectively, addressing significant production challenges in the biotechnology and life sciences industries.
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