APEX platform for genome-wide stabilization trait discovery in gram negative bacteria

Overview

Firelight Biosciences introduces its APEX platform, a cutting-edge technology designed for the genome-wide exploration of stabilization traits in Gram Negative bacteria. By leveraging high-throughput, parallel genome editing, the platform identifies and optimizes genetic sources of cell stability and viability. This enables the development of microbial strains with enhanced tolerance to stresses such as heat-shock and desiccation. The APEX platform serves as a powerful tool for industries aiming to develop robust microorganisms for various applications.

Technical specifications

The APEX platform operates through:

• High-throughput, parallel genome editing: Capable of editing all loci across the genome, both native and introduced, to discover beneficial genetic changes.
• Targeted edits: Includes gene knockout and insertion of promoters with varying strengths to assess impact on stress tolerance.
• Population-based selection: Strains are subjected to growth challenges to identify edits enhancing performance.
• Deep sequencing analysis: Utilizes sequencing to pinpoint protective proteins and enriched edits for further validation.

Technology readiness level

The APEX platform is currently at TRL 3, indicating successful proof of concept. Future developments include collaboration with sponsors to engineer E. coli strains expressing candidate heterologous cryoprotective proteins and further validation under selective conditions.