Arms-based SNP detection for micronutrient deficiency risk

Overview

The Amplification-refractory mutation system (ARMS)-based SNP detection technology offers a rapid, cost-effective, and scalable solution for identifying single nucleotide polymorphisms (SNPs) associated with micronutrient deficiency risks. Utilizing a PCR-based method, it allows for direct detection and differentiation of homozygous wildtype, homozygous mutated, and heterozygous genotypes in a single reaction, with results available in less than 6 hours. This technology is ideal for high-throughput screening applications, particularly in resource-limited settings.

Technical specifications

• Cost-effective: The genomic DNA extraction and SNP detection process costs less than $1 per sample, significantly reducing testing expenses compared to commercial alternatives.
• Rapid results: Capable of processing over 20 samples within a 6-hour timeframe, making it suitable for both commercial and clinical applications.
• High accuracy: Demonstrated 100% accuracy in SNP detection in animal models, validated through sequencing.
• Scalability: Designed to accommodate high-throughput production needs, enhancing its utility in large-scale screening programs.
• Broad application potential: Initially tested in mouse and rat models, with plans to validate using human samples for SNPs linked to conditions such as sickle cell anemia.

Technology readiness level

This technology is currently at TRL 5, having been successfully tested in relevant animal models. Future validation steps include using human samples, particularly those containing SNPs associated with micronutrient deficiencies, to further demonstrate its applicability and reliability.