Automated sample preparation system for ddpcr-based pathogen quantification
An integrated automated system for droplet digital PCR (ddPCR) that enhances pathogen quantification by improving sample purity and recovery efficiency from diverse food matrices. Features rapid processing and compatibility with ddPCR workflows.
Overview
The automated sample preparation system is designed to support droplet digital PCR (ddPCR) applications by improving the quantification of low-abundance pathogens in complex food matrices. This system integrates vacuum-assisted filtration, magnetic bead-based nucleic acid extraction, and microfluidic reagent handling to achieve high DNA recovery efficiency (>95%) and purity, crucial for ddPCR accuracy. It processes samples in under 3 hours, delivering highly concentrated DNA suitable for ddPCR analysis, making it a valuable tool for food safety testing.
Technical specifications
- Vacuum-driven microfiltration captures microorganisms while excluding debris, enhancing sample purity.
- Programmable magnetic capture utilizes bead-based nucleic acid extraction to ensure high recovery rates.
- Microfluidic reagent handling allows for precise buffer loading and washing, removing PCR inhibitors like polysaccharides and heme.
- Compact and modular design facilitates automation and parallel processing, adaptable to various lab scales.
- Rapid processing time of <3 hours for volumes up to 250 mL, resulting in DNA eluates of <100 µL.
Technology readiness level
Currently at TRL 4, the system has been prototyped and is undergoing further validation with a commercial instrumentation partner. Future work involves optimizing buffer chemistries and magnetic bead formulations for compatibility with commercial ddPCR workflows, aiming for a standardized, cartridge-based module suitable for large-scale deployment.
About Sungkyunkwan University
Sungkyunkwan University (SKKU) is a comprehensive private research university with campuses in Seoul and Suwon, pairing deep heritage with a modern, industry‑oriented culture. Proximity to Samsung’s R&D and manufacturing hub and extensive on‑campus labs enable direct pathways for sponsored research, contract testing, and joint development. The medical school’s integration with a major tertiary hospital in Seoul provides access to clinical trials and device validation, while an industry–university cooperation foundation streamlines agreements and IP. Research is supported by competitive national funding from the National Research Foundation of Korea and relevant ministries, and a dedicated technology transfer office advances licensing and startup formation.
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