Biophysical analysis of cuticular permeability for systemic fungicides in berry crops

Technology
Conceptual
University

This research investigates how environmental factors like temperature and humidity affect the cuticular membrane's permeability to systemic fungicides in berry crops, utilizing advanced analytical techniques to optimize fungicide application.

Overview

This innovative research explores how environmental stresses impact the permeability of plant cuticular membranes (CMs) to systemic fungicides, focusing on berry crops. By understanding these biophysical factors, the study aims to enhance the efficiency of fungicide applications, potentially leading to more sustainable agricultural practices and improved crop protection.

Technical specifications

Key methodologies:

  • Quantitative analytical workflow: Combines gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID) to analyze chemical changes in CMs due to environmental factors.
  • Microscopy techniques: Utilizes cryo-scanning electron microscopy and high-resolution confocal Raman microscopy to examine the structural features and wax deposition patterns in CMs.
  • CM isolation protocols: Developed to determine transport parameters such as CM permeances and the transport kinetics of systemic fungicides.
  • Fungicide accumulation measurement: Employs QuEChERS extraction protocols and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for accurate quantification of fungicide residues in berry tissues.
Technology readiness level

The current research is at Technology Readiness Level 2, indicating that the concept has been formulated and principles have been observed. Further validation is planned through detailed studies on the effects of temperature and humidity on CM parameters in five berry species.


About University of British Columbia

UBC is a comprehensive public research university with major campuses in Vancouver and the Okanagan, among Canada’s largest and most internationally connected. Industry engages through a co-op talent pipeline and collaborative research spaces that include co-located clinical settings and on‑campus testbeds. A dedicated technology transfer office brokers partnerships, manages IP and licensing, and is complemented by an active venture accelerator and incubator network. Research is supported by competitive funding from Canada’s Tri‑Council agencies (NSERC, CIHR, SSHRC) and infrastructure investments from the Canada Foundation for Innovation, alongside provincial and industry sponsorship.

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