BTSL protein knockout to enhance iron and zinc in tomatoes for improved nutrition
This project aims to biofortify tomatoes by knocking out BTSL proteins, enhancing iron and zinc content to address deficiencies affecting over 2 billion people. Using CRISPR/Cas9 technology, the research targets improved nutritional profiles in tomato consumption.
Overview
Addressing global micronutrient deficiencies, this research focuses on biofortifying tomatoes to increase their iron (Fe) and zinc (Zn) content. By knocking out BRUTUS-LIKE1 (BTSL1/2) proteins using CRISPR/Cas9 gene editing, the project aims to enhance Fe and Zn uptake in tomato plants. The goal is to improve the nutritional status of populations reliant on cereal-based diets, which are typically low in bioavailable Fe and Zn.
Technical specifications
- CRISPR/Cas9 Gene Editing: Utilizes CRISPR/Cas9 constructs targeting BTSL genes to knock out the proteins responsible for Fe/Zn homeostasis.
- Transformation Pipeline: Employs Agrobacterium-mediated transformation for introducing gene edits into tomato cultivar Moneymaker.
- Phenotype and Genotype Screening: Edited plants are screened via molecular genotyping and sequencing to confirm successful edits.
- Nutrient Measurement: Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) is used to measure Fe/Zn concentrations in edited and wild-type plants.
Technology readiness level
The project is currently at Technology Readiness Level 2, involving the early proof of concept stage with CRISPR/Cas9 constructs being designed and transformation protocols being established for further validation.
About Danforth Plant Science Center
The Donald Danforth Plant Science Center is a leading independent, nonprofit research institute in St. Louis with a multidisciplinary research community focused on plant science. Corporate engagement is built into the campus through co‑located labs, flexible leased space, and shared core facilities that enable side‑by‑side collaboration with startups and established R&D teams. Its location within a regional agtech innovation district places partners near major corporate hubs, venture networks, and field‑to‑lab testing resources. Research is supported by competitive federal funding from agencies such as the NSF, USDA, and DOE, alongside industry‑sponsored projects and philanthropy. A dedicated technology transfer office provides IP management, licensing, and venture formation support.
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