Clia-based microfluidic chip for detecting tobamoviruses in plants
A microfluidic chip solution combining CLIA and advanced sensors to detect viable tobamoviruses in plant samples with PCR-like sensitivity. It offers rapid, reliable results and can adapt to various plant species and viruses.
Overview
The CLIA-based microfluidic chip developed by the Fraunhofer Institute for Microengineering and Microsystems offers a cutting-edge solution for detecting viable tobamoviruses directly in plant seeds and cells. Utilizing chemiluminescence immunoassays on a microfluidic platform, it provides rapid and highly sensitive detection of virus-specific proteins, achieving sensitivity comparable to PCR. This system is designed to confirm the presence of infectious viruses by observing increased viral protein concentrations, ensuring early and reliable virus identification.
Technical specifications
The technology integrates microfluidics with chemiluminescence detection and single-photon avalanche diodes to enhance sensitivity and reliability. The automated on-chip analysis allows for quick turnaround times and consistent results. The microfluidic chip's flexibility enables it to be easily adapted for different plant species and viruses, allowing for broad application potential. Key features include:
- Automated CLIA on microfluidic chip
- Detection of virus-specific proteins with enhanced sensitivity
- Adaptability for various plant species and viruses
- High degree of automation for improved reproducibility
Technology readiness level
Currently at TRL 3, this technology has been demonstrated in a laboratory setting. Future validation efforts include collaborating with partners to select target organisms and antibodies, integrating immunoassays into the chip, and optimizing bioassay processes. The aim is to enhance workflow efficiency and detection sensitivity, with proof of concept expected within 12 to 18 months.
About Fraunhofer Institute
Fraunhofer is a large, multi-site applied research organization based in Germany, operating a nationwide network of institutes and research units with a strong industry-facing mission. Its contract-research model aligns work to real manufacturing and deployment needs, with pilot lines, test labs, and demonstration facilities for validation and scale-up. Many institutes are integrated with nearby universities and regional industry clusters, enabling joint appointments, shared infrastructure, and fast talent pipelines. Research is supported by competitive European programs alongside German federal and state funding, complemented by extensive contract revenue from private-sector collaborations. A dedicated technology transfer function manages IP, licensing, and startup formation.
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