Crispr-based genomic reorganization for tandem expressed gene pairs in crop plants

Overview

This offering provides expert CRISPR-based genomic reorganization capabilities for crop plants, with a focus on creating tandem expressed gene pairs to manipulate secondary metabolism. The laboratory brings decades of genetic engineering experience to design guide RNAs, identify transgenes, and execute precision genome edits tailored to the target plant species. The approach is adaptable across plant systems and is informed by ongoing evaluation of CRISPR-edited plants for gene expression differences.

Technical specifications

• CRISPR-based gene editing for targeted manipulation of plant secondary metabolism pathways
• Guide RNA design and transgene identification guided by 35+ years of genetic engineering expertise
• Genomic reorganization to create tandem expressed gene pairs, enabling coordinated gene expression
• Active validation pipeline with CRISPR-edited plants currently being evaluated for gene expression differences
• Adaptable methodology that can be applied across multiple crop plant species depending on ploidy, gene copy number, and available genomic information

Technology readiness level

The laboratory is actively conducting basic research with CRISPR-edited plants currently under evaluation for gene expression differences. The core competencies in guide RNA design, transgene identification, and genetic engineering are well established through decades of prior work. Future validation steps depend on the specific gene targets and genomic architecture of the plant system of interest, meaning the readiness level is best characterized as early-stage research with proven enabling expertise ready to be applied to new targets.