CRISPR fusion directed DNA methylation control in plants

Overview

The CRISPR fusion directed DNA methylation control platform provides a novel approach to plant gene expression management. By employing CRISPR-ROS1 and related dCas fusion systems, this technology allows for the precise modification of DNA methylation at specific plant loci. This enables programmable gene expression changes without permanently altering the DNA sequence itself. The platform leverages Solis Agrosciences' advanced protoplast systems to rapidly screen and validate epigenetic constructs, guide RNA activity, and methylation changes before moving to stable plant transformations. This significantly accelerates development timelines and reduces technical risks and costs.

Technical specifications

• Utilizes CRISPR-ROS1 and dCas fusion systems for targeted DNA methylation
• Advanced protoplast systems for rapid transient screening
• Capable of modifying gene expression without permanent DNA changes
• Supports multiple crop species through plant transformation and regeneration
• Comprehensive molecular validation and phenotypic outcome assessments

Technology readiness level

The technology is at TRL 4, indicating that it has been validated in a laboratory setting. Future efforts will focus on optimizing CRISPR-demethylase fusion strategies and conducting pilot trials to establish its efficacy across various plant species.