CRISPR-guided targeted integration platform for large DNA cassettes in crops
A targeted DNA integration platform combining programmable CRISPR/Cas genome targeting with site-specific integration mechanisms to enable precise insertion of multi-kilobase genetic cassettes in crops. Designed to reduce positional effects, simplify breeding, and support predictable trait development in species such as Brassica/oilseed rape and soybean.
Overview
This offering is a precision genome engineering platform that enables targeted insertion of large DNA cassettes into crop genomes. By combining programmable CRISPR/Cas-mediated targeting with site-specific DNA integration, the platform addresses the limitations of conventional random integration and homology-dependent repair for large payloads. The result is more predictable trait development, reduced positional effects, and simplified downstream breeding and molecular characterization.
The platform is being developed and optimized in commercially important crops, with current focus on Brassica/oilseed rape and soybean, and is intended to scale toward 15–25 kb payloads suitable for complex trait stacks.
Technical specifications
Key features:
- Programmable CRISPR/Cas targeting combined with site-specific integration mechanisms for precise insertion of multi-kilobase cassettes
- Integrated workflow covering target-site design, large-DNA donor delivery, efficient integration, and optimized transformation and regeneration
- Candidate event screening using PCR, sequencing, copy-number analysis, and molecular characterization to identify full-length, correctly targeted, preferably single-copy insertions
- Proof-of-concept insertion demonstrated with 7–10 kb DNA payloads
- Optimization of transformation and regeneration pipelines for difficult crop backgrounds, including Brassica/oilseed rape and soybean
- Capability development toward 15–25 kb payloads for advanced trait stacks
Underlying expertise:
- CRISPR/Cas9 editing and gene overexpression
- Molecular cloning and construct design
- Plant transformation, tissue culture, and protoplast regeneration
- Molecular validation and characterization
Technology readiness level
The platform is at an early-to-mid development stage. A five-step validation roadmap is in place: design of target sites, CRISPR components, and donor constructs; proof-of-concept insertion of 7–10 kb DNA; optimization of transformation and regeneration in target crops; validation of precise, full-length, single-copy insertion using PCR and sequencing; and assessment of stable inheritance with extension toward 15–25 kb payloads. Current readiness supports collaborative development and sponsored research, with further validation and scale-up planned.
About SEJONG UNIVERSITY
Sejong University is a comprehensive private research university in Seoul, South Korea, with a practical, industry-facing culture. Corporate partners engage through collaborative labs and contract research facilitated by an industry-academic cooperation foundation that streamlines agreements and compliance. A central Seoul location puts talent next to major technology and manufacturing hubs, enabling internships, executive education, and fast site-to-lab collaboration. Research is backed by competitive national programs, including the National Research Foundation of Korea and government ministries, and a dedicated technology transfer office manages IP, licensing, and startup support.
delivered to your inbox.