DNRA Bacterial Technology for Organic Fertilizers to Reduce Leaching and Emissions
NoN2O is an innovation project developing a microbial biofertilizer additive that utilizes the DNRA pathway to convert nitrate into soil-binding ammonium. This technology reduces agricultural nitrate leaching and nitrous oxide emissions while improving nitrogen use efficiency, providing a sustainable, transportable solution for modern farming.
Overview
The NoN2O project is a five-year initiative developing a specialized microbial biotechnology designed to mitigate the environmental impacts of nitrogen fertilizers in agriculture. By utilizing specific soil-derived microorganisms capable of the Dissimilatory Nitrate Reduction to Ammonium (DNRA) pathway, this solution addresses two critical challenges: nitrate leaching into water systems and the emission of nitrous oxide (N2O), a potent greenhouse gas. The technology is being developed for integration into organic fertilizers, offering a sustainable approach to enhance nitrogen efficiency while protecting ecosystems.
Technical specifications
The technology focuses on a dry, organic fertilizer formulation infused with identified DNRA-capable bacteria. Key functional capabilities include:
- Conversion of nitrate (NO3-) into ammonium (NH4+) via the DNRA process, which reduces nitrogen loss because ammonium binds to soil particles.
- Competitive inhibition of N2O-producing microorganisms by reducing nitrate availability in the soil.
- Direct biological conversion of nitrous oxide (N2O) into inert nitrogen gas (N2).
- Development of a dry product format to ensure long shelf life, ease of storage, and improved transportability compared to liquid alternatives.
Technology readiness level
The technology is in the early research and development phase, with a project duration spanning from 2025 to 2030. Current activities focus on laboratory-based identification and characterization of suitable microorganisms, followed by greenhouse and field trials. As of the current project stage, it is classified as TRL 3, moving toward TRL 4 as it transitions from initial proof-of-concept research to lab-based validation and subsequent prototype testing.
About Danish Technological Institute
Danish Technological Institute is an independent, non‑profit research and technology organization of national scope. Headquartered in Taastrup with facilities across Denmark, it provides applied R&D, prototyping, and accredited testing and certification tightly aligned to industry needs. Co‑located pilot and demonstration facilities, industrial testbeds, and training programs enable partners to validate, scale, and adopt new technologies under real‑world conditions. Research and innovation activities are supported by competitive Danish and European funding, including national innovation programs and EU framework initiatives. A dedicated commercialization function supports IP strategy, licensing, and collaborative agreements.
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