Dsrna seed treatment for sustainable control of soybean sudden death syndrome

Overview

The dsRNA-based seed treatment developed by HarvestR targets Fusarium virguliforme, the pathogen causing Sudden Death Syndrome (SDS) in soybeans. Utilizing RNA interference, this solution silences essential genes of the pathogen, offering a targeted, environmentally friendly alternative to traditional fungicides. This innovative method leaves no chemical residues and minimizes resistance risk, aligning with global trends in sustainable agriculture. It can be seamlessly integrated with existing fungicide treatments, providing growers with an effective tool to manage SDS while adhering to safety and sustainability standards.

Technical specifications

Key features:

• Utilizes double-stranded RNA (dsRNA) to trigger gene silencing in pathogens
• Targets essential genes of Fusarium virguliforme without affecting soybeans or non-target organisms
• Compatible with standard fungicide seed coatings
• Formulated as a stable seed coating for low-volume application
• Proven efficacy against similar pathogens like Botrytis, Penicillium, and Colletotrichum

Application process:

• Phase 1: Identify fungal targets with no homology to soybean genes
• Phase 2: Synthesize and screen dsRNAs for gene silencing and pathogen growth inhibition
• Phase 3: Optimize dsRNA stability and delivery in seed coating matrices
• Phase 4: Test under greenhouse conditions for uptake, persistence, and disease suppression

Technology readiness level

The dsRNA seed treatment is currently at Technology Readiness Level 3, with ongoing validation phases designed to enhance its efficacy and compatibility with commercial protocols.