Enhanced CAR-T cell migration for improved tumor targeting
This research offers a breakthrough in CAR-T cell therapy by identifying molecular mechanisms to enhance tumor-specific migration, reducing off-target effects and toxicity. Aimed at treating solid tumors more effectively, the study uses CRISPR–Cas9 and pharmacologic screens to identify key migration mediators.
Overview
This innovative research addresses the challenge of improving CAR-T cell therapy for solid tumors by focusing on enhancing the tumor-specific migration of these cells. The study aims to identify molecular mechanisms that prevent off-target CAR-T cell sequestration, thereby improving efficacy and reducing the risks of nonspecific toxicity. This is critical as solid tumors represent 90% of adult human cancers and effective targeting remains a significant unmet clinical need.
Technical specifications
The research employs cutting-edge techniques including:
- In vivo CRISPR–Cas9 knockout screen: Used to identify key T cell-intrinsic mechanisms that regulate CD8 T cell migration.
- High-throughput pharmacologic screening: Aimed at discovering pathways that enhance CAR-T cell targeting.
- Focus on St3gal1 inhibition: Testing its role in reducing cytokine secretion and pulmonary vascular damage by preventing nonspecific CAR-T cell sequestration.
- Examination of BLT1 pathway: Investigating its potential as a universally conserved CAR-T cell-intrinsic program for tumor-specific migration.
Technology readiness level
The technology is currently at TRL 4, indicating it has been validated in a laboratory setting and is moving towards further development and testing.
About University of Rochester
The University of Rochester is a private research university in upstate New York anchored by an integrated academic medical center. Industry partners access co-located clinical and engineering labs, shared core facilities for imaging, materials, analytics, and prototyping, and large-scale laser and high‑energy‑density testbeds. Its position within a long-standing regional optics and photonics ecosystem enables rapid teaming with suppliers, startups, and established firms for sponsored research and product development. Research is supported by competitive federal funding, including NIH, NSF, DOE, and DoD. A dedicated technology transfer office streamlines IP, licensing, and startup formation and links companies to regional incubators and accelerators.
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