Enzyme-enhanced gluten network for al dente shelf-stable pasta
This research explores the use of enzymes and additives to strengthen the gluten network in pasta, aiming to maintain its al dente texture in shelf-stable products. Key enzymes include transglutaminase, glucose oxidase, and phospholipase, with additives like ascorbic acid.
Overview
This innovative research initiative aims to enhance the al dente texture of shelf-stable pasta using a combination of enzymes and additives. By strengthening the gluten network in flour dough during pasta processing, this solution seeks to maintain the desired texture even after long storage periods. The approach leverages enzymes such as transglutaminase, glucose oxidase, and phospholipase, as well as additives like ascorbic acid, azodicarbonamide, and diacetyl tartaric acid esters of mono- and diglycerides to achieve optimal results.
Technical specifications
Key enzymes and additives:
- Enzymes: Transglutaminase (TG), Glucose oxidase (GOX), Phospholipase (PHOS)
- Additives: Ascorbic acid (AA), Azodicarbonamide (ADA), Diacetyl tartaric acid esters of mono- and diglycerides (DATEM)
Experimental approach:
- Utilize a Plackett Burmann experimental design to determine effective enzyme and additive combinations
- Conduct texture analysis using a TA-XT2 texture analyzer
- Evaluate pasta texture, color, water activity, and cooking quality over a 60-day period
Technology readiness level
The technology is currently at TRL 2, indicating the concept has been formulated and initial experimental designs are in place. Over the next six months, further validation through lab-based experimentation and sensory analysis trials is planned to advance the readiness level.
About Universidade Estadual de Campinas
Universidade Estadual de Campinas (Unicamp) is a comprehensive public research university in Campinas, São Paulo, recognized for high research intensity and strong graduate education. Industry engages through an on‑campus science and technology park with co‑located corporate R&D suites and shared core facilities. Clinical translation is enabled by an integrated teaching hospital and specialized centers, while proximity to the Campinas–São Paulo innovation corridor makes onsite collaboration and talent access straightforward. Research is supported by state and federal agencies such as FAPESP, CNPq, CAPES, and FINEP, alongside industry contracts. A dedicated technology transfer office manages IP, licensing, and startup formation, and offers clear pathways for NDAs, sponsored research, and scale‑up.
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