Epigenetic platform for chromatin-based stress memory in crops

Overview

The proposed solution is an advanced epigenetic platform designed to enhance crop resilience by triggering chromatin-based stress memory. Using exogenous synthetic dsRNAs, this platform mimics natural plant epigenetic switches to modulate chromatin topology, priming stress-responsive gene expression without altering DNA sequences. Unlike existing RNA technologies targeting pathogen RNAs, this approach strengthens the plant's innate resilience, offering a transgene-free and field-deployable solution. This innovation is particularly valuable for polyploid crops where conventional genome editing is challenging.

Technical specifications

Key features:

• Exogenous delivery of synthetic dsRNAs that mimic natural epigenetic switches
• Induces locus-specific DNA methylation and chromatin remodeling
• Promotes faster and stronger activation of stress-responsive genes without genomic modification
• Leverages established nuclear uptake mechanisms and field-compatible delivery platforms
• Non-GMO solution aligning with global regulatory trends
• Initial focus on enhancing heat stress tolerance in tomatoes, with potential expansion to other stress conditions and crops

Technology readiness level

The technology is currently at TRL 4, having demonstrated successful molecular validation in various species. The project aims to reach TRL 5 through further in planta validation and development of a pre-commercial prototype, including greenhouse trials to measure epigenetic modifications and stress tolerance improvements.