Four-channel CARS imaging for non-destructive ploidy detection in wheat embryos

Overview

This solution leverages four-channel Coherent Anti-Stokes Raman Scattering (CARS) imaging to provide a label-free, non-destructive method for early ploidy detection in wheat embryos. By capturing specific DNA and protein signals, the technique delivers a rapid and accurate binary classification of ploidy levels—"2N – Keep" or "1N"—while preserving over 95% of embryo viability. The approach is designed to align with wheat doubled haploid (DH) production workflows and to scale up to hundreds of embryos per day without the use of restricted materials.

Technical specifications

• Four-channel imaging: Captures DNA-specific signals at 788 cm⁻¹, chromatin at 2930 cm⁻¹, protein reference at 1650 cm⁻¹, and background at 2850 cm⁻¹.
• High throughput: Capable of processing 500–2,000 embryos per day.
• Automation: Includes software for automatic nuclei segmentation and DNA/protein ratio computation.
• Non-destructive: Imaging is performed on intact living embryos, ensuring high viability.
• Compatibility: Integrates with existing wheat DH production processes.

Technology readiness level

Currently at TRL 3, the solution has completed proof-of-concept and is undergoing validation with wheat protocols. Future phases include blind testing and high-throughput scale-up, with a target to reach TRL 5 through joint testing and integration with relevant teams.