Fungal artificial chromosome technology for novel food preservative discovery
Revolutionary fungal artificial chromosome (FAC) technology for discovering novel food preservatives by upregulating gene expression in diverse fungi, enabling high-throughput screening of small molecular compounds and proteins.
Overview
The fungal artificial chromosome (FAC) technology is an innovative approach designed to transform the discovery of food preservatives through rapid and efficient screening of novel small molecular compounds and proteins. This patented 2nd generation FAC technology allows for the upregulation of approximately 100kb DNA fragments within a single well, facilitating the heterologous expression of genes from a filamentous fungus. With over 20 FAC libraries derived from diverse fungi, the platform is primed for large-scale screening to identify compounds with potential antifungal properties, which could revolutionize the food preservation industry.
Technical specifications
- FAC Libraries: Over 20 libraries available, providing extensive genetic diversity for screening.
- High-throughput Screening: Conducted in a 96-well format for simultaneous discovery of multiple compounds.
- Compound Production: Capable of producing high titers of natural ingredients such as fungal organic acids, fermentation byproducts, and antifungal peptides.
- Assay Targets: Includes Zygosaccharomyces bailii and other yeast species for growth inhibition or killing assays.
Technology readiness level
The technology has reached a Technology Readiness Level (TRL) of 8, indicating that it has been tested in its final form and under expected conditions. Current and future validation efforts include screening multiple fungi for novel natural ingredients and conducting growth inhibition assays in collaboration with industry partners.
About Intact Genomics
Intact Genomics is an ISO 13485:2016 certified biotechnology company that specializes in the development and manufacture of high-performance molecular biology reagents and genomic services. Founded in 2013 and based in St. Louis, Missouri, the company leverages proprietary technologies, including Fungal Artificial Chromosomes (FAC) and advanced large DNA fragment cloning, to provide solutions for isothermal DNA/RNA amplification, gene editing, and molecular cloning. Their product portfolio, which includes over 100 items such as high-efficiency competent cells, enzymes, and RPA optimization tools, is designed to support high-quality, reproducible research across diverse fields including human health, agriculture, and environmental science.
The company serves a global customer base across academia and industry, enabling researchers to address complex challenges in genomic structure, function, and natural product discovery. By offering both off-the-shelf molecular biology products and custom services—such as library construction, protein expression, and OEM manufacturing—Intact Genomics provides scalable support for diagnostic development and drug discovery efforts. Their commitment to reliability and innovation has established them as a key resource for laboratories seeking specialized tools for streamlined cloning and rapid isothermal testing methods.
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