Gene editing solution for reducing toxic SGA content in potatoes
A novel approach utilizing phytochrome interacting factors (PIFs) to downregulate toxic steroidal glycoalkaloid (SGA) levels in potatoes, enhancing nutritional value. This gene editing method targets SGA biosynthesis pathways to produce safer, healthier potato cultivars.
Overview
This innovative solution targets the reduction of toxic steroidal glycoalkaloid (SGA) content in potatoes through gene editing, specifically focusing on phytochrome interacting factors (PIFs). This approach aims to enhance the nutritional quality of potatoes by decreasing levels of α-chaconine and α-solanine, which are harmful to humans at high concentrations. By modulating the SGA biosynthesis pathway, the research seeks to develop new potato cultivars that are safer for consumption while maintaining their economic and nutritional importance.
Technical specifications
- Target Genes: PIFs in Solanum tuberosum L. regulate SGA biosynthesis by interacting with the promoter regions of SGA-associated genes.
- Gene Editing Technique: Utilizes CRISPR vectors (pCAMBIA1305/pKSE401) for cloning and transformation in Rhizobium radiobacter and subsequent potato transformation.
- Validation Methods: Includes docking studies, in vivo assessments, qRT-PCR for gene expression analysis, CHIP assays for confirming gene interactions, and HPLC for measuring SGA content.
Technology readiness level
This solution is at Technology Readiness Level 3, with identified and characterized PIFs undergoing validation in laboratory settings. Further development and testing are planned to confirm the efficacy and safety of the modified potato lines.
About Panjab University
Panjab University is a comprehensive public research university in Chandigarh, India, serving a large and diverse student body. Industry engagement is enabled by coordinated liaison and placement offices that structure internships, sponsored projects, and consulting, plus shared instrumentation facilities for contract testing and prototyping. Its location in the Chandigarh–Mohali–Panchkula tri-city, near the Baddi manufacturing hub, positions partners close to suppliers, national institutes, and a deep talent pool. Research is supported by competitive funding from Indian agencies such as DST, DBT, CSIR, and ICMR, with established IP policies and entrepreneurship support to streamline collaboration and licensing.
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