Gene expression fine-tuning using promoter variants in rice
This research explores the use of promoter variants to fine-tune gene expression in rice, leveraging genomic data from the 3000 Rice Genomes Project. It aims to establish causal links between promoter variants and gene expression changes to improve rice traits.
Overview
This research initiative focuses on refining gene expression in rice through the identification and functional characterization of promoter variants. By utilizing the extensive genomic data from the 3000 Rice Genomes Project, the research targets genes with significant promoter variants that show high allele frequencies and are linked to observable phenotypic changes, such as variations in plant height and leaf color. The goal is to understand how specific promoter variants influence gene expression and, consequently, rice plant traits, thereby offering potential pathways for crop improvement.
Technical specifications
Research Methods:
- Identification of target genes with significant promoter variants.
- Functional characterization by comparing gene expression levels and phenotypes in rice accessions with different allelic variants.
- Use of reporter constructs (YFP or LUC) to quantify gene expression changes through transient expression assays in protoplasts or stable transformation in rice plants.
- Future plans include haplotype analysis and comparison of gene expression across different haplotypes.
Applications:
- Potential enhancement of rice traits such as plant height and leaf color through precise gene expression modulation.
- Broader implications for crop improvement strategies in agriculture by exploiting natural genetic variation.
Technology readiness level
The technology is at TRL 4, indicating that the research is in the early stages of validation with some laboratory-based validation completed. Further validation and optimization are planned through future experiments, including transgenic rice plants and haplotype analysis.
About Shanghai Jiao Tong University
Shanghai Jiao Tong University is a comprehensive public research university in Shanghai and one of China’s longest-established institutions. Companies engage through co-located enterprise joint labs and shared engineering platforms, plus a research and technology park that supports prototyping and pilot work. The medical school and affiliated hospitals enable large-scale clinical studies and clinical translation, while proximity to Shanghai’s advanced manufacturing and biopharma clusters connects partners to robust supply chains and testbeds. Research is supported by competitive national and municipal funding, including programs from major science agencies. A dedicated technology transfer office provides IP management, licensing, startup support, and streamlined contracting for sponsors.
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