Genome-edited sweeter tomatoes with low glucose index
Developing a new tomato variety with enhanced sweetness and low glucose index using RNP-mediated genome editing. This innovation leverages the high fructose content of wild tomato species to improve the flavor profile of cultivated tomatoes, addressing consumer demand for tastier produce.
Overview
This project aims to enhance the sweetness of cultivated tomatoes by increasing their fructose content while maintaining a low glucose index. Leveraging the natural high fructose-to-glucose ratio found in the wild tomato species LA1036, the research utilizes genome editing techniques to integrate these desirable traits into commercial tomato varieties. The result will be a sweeter tomato that meets consumer demands for better-tasting produce without compromising on health attributes.
Technical specifications
The research employs RNP-mediated genome editing to modify the sugar profile of cultivated tomatoes. Key steps include:
- Comparative analysis: Using PacBio sequencing and RNA sequencing to identify candidate genes responsible for high fructose content in LA1036.
- Genetic mapping: Conducting QTL and association analysis to pinpoint genetic markers correlated with desired sugar content.
- Genome editing: Applying RNP-mediated techniques to integrate identified genes into elite germplasm.
- Validation: Developing and employing an ELISA test for accurate measurement of sugar content in modified tomatoes.
Technology readiness level
The project is currently at TRL 2. Initial laboratory experiments have been conducted, focusing on identifying genetic candidates and conducting preliminary genome edits. Future steps involve comprehensive validation and scaling of the genome editing process to ensure successful integration of traits into commercial varieties.
About lark seeds international
Founded in 1997, Lark Seeds International is a global organization specializing in the research, breeding, marketing, and distribution of premium hybrid vegetable seeds. The company leverages advanced agricultural technology, including data analytics and its proprietary Advantage+ platform—which utilizes next-generation breeding tools such as CRISPR/RNP—to develop varieties that address complex industry challenges. Its research focuses on creating sustainable solutions that provide high yields, disease resistance, and desirable commercial attributes for diverse environments, with breeding facilities and trial stations distributed across the United States, Canada, Mexico, Europe, the Middle East, North Africa, and South America.
This work is critical for growers, processors, and end-consumers who face pressures from changing environmental conditions, climate change, and specific agricultural pests like branched broomrape. By collaborating closely with clients to understand local market needs, the company aims to mitigate grower risk and provide competitive products. Recent milestones include alliances with major partners like Bayer to deploy genome-editing technologies and the development of new tomato hybrids designed to withstand parasitic weed infestations, demonstrating its commitment to elevating nutrition, flavor, and harvest efficiency globally.
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