Genomic resources for locally adapted entomopathogenic nematodes for precision biocontrol

Overview

This program generates genomic resources for locally adapted entomopathogenic nematodes (EPNs), which are biological control agents used to suppress insect pests in agricultural settings. Commercially available EPNs often suffer high mortality after field application because they are poorly adapted to local environmental conditions, making biocontrol financially unattractive for growers. By identifying and genetically characterizing EPN strains that are naturally adapted to specific agricultural environments, this research enables precision breeding and targeted genetic engineering to produce hardier, more effective biocontrol agents. The result is improved pest control efficacy, reduced EPN production costs, and a more economically viable alternative to broad-spectrum chemical pesticides.

Technical specifications

Research approach:

• Systematic soil sampling across diverse agricultural land types in Alberta, Canada, to isolate locally adapted EPN strains
• Metabarcoding of the ITS2 region for genetic identification of nematode genera and species
• Reference genome assembly and gene model generation for all newly discovered EPN species
• Population-level resequencing of locally adapted strains of known species to identify genomic regions associated with environmental adaptation
• Comparative survival and pathogenicity testing of isolated strains against commercially available EPNs using local crops and target pathogens

Key features:

• Preliminary data indicates EPN presence in approximately 10% of Prairie soil samples, with at least one likely new species confirmed via genetic barcoding
• Novel genetic material not represented in existing public databases, indicating unique biodiversity value
• Genome assembly and annotation partnerships already established for downstream characterization
• Focus on linking specific genomic variants to survival traits and pest-control performance under real-world agricultural conditions

Technology readiness level

The program is currently at an early-to-mid discovery stage. Preliminary sampling and genetic barcoding have been completed, confirming the presence of locally adapted EPNs and at least one putatively new species. Ongoing work is validating survival rates and pathogenicity of isolated strains relative to commercial benchmarks. Reference genome assembly and annotation are pending confirmation of DNA results, with service agreements already in place. Future validation will expand sampling geographically across Alberta, generate complete reference genomes for new species, and identify adaptive genomic regions through population resequencing. The technology readiness level is appropriate for collaborative research partnerships and sponsored development aimed at translating genomic discoveries into commercial biocontrol products.