Hemoprint - molecular fingerprinting of capillary blood RNA

Overview

HemoPrint is a cutting-edge platform developed at Ghent University, designed for low-cost, scalable RNA profiling from self-collected capillary blood. This innovative solution allows individuals to use a simple finger prick to collect a minute blood sample, which can be easily shipped to a central lab for analysis. HemoPrint is particularly valuable for longitudinal health monitoring, as it can reveal shifts in blood RNA signatures associated with micronutrient levels, offering a personalized approach to health management.

Technical specifications

• Sample Collection: A lancet is used for a finger prick to collect 30 µL of capillary blood, stabilized in a microsampling device.
• Stability: The collected sample is stable for 2 months when refrigerated and 2 weeks at room temperature, supporting flexible shipment.
• Analysis Process: In the lab, RNA is extracted and prepared for sequencing in a 96-well format, focusing on the 3' end of polyadenylated genes.
• Data Output: Quantification of the top 6000 abundant genes, along with differential gene expression, pathway analysis, gene set enrichment, molecular clock analysis, and immune cell enumeration.
• Cost Efficiency: The platform operates at a low two-digit cost, making it accessible for large-scale use.

Technology readiness level

HemoPrint is currently at Technology Readiness Level 5. The platform is operational, and future validation will focus on profiling donors with known micronutrient levels to establish predictive models for health states, further enhancing its utility in personalized health diagnostics.