High-throughput promoter sequence testing in plants
A high-throughput platform for testing promoter sequences in plants using advanced cloning and transformation systems. This technology allows for the analysis of promoter activity and SNP effects using transient expression assays, facilitating plant synthetic biology research.
Overview
This solution offers a high-throughput platform for testing and analyzing promoter sequences in plants, utilizing advanced cloning and plant transformation systems. The technology enables researchers to assess how specific promoter sequences and single nucleotide polymorphisms (SNPs) affect gene expression. By leveraging transient expression assays in Nicotiana benthamiana, combined with dual-luciferase or luciferase-GUS assays, this platform supports the identification and functional testing of promoters. This approach is particularly valuable for plant synthetic biology applications, allowing for the exploration of natural diversity or the creation of synthetic promoters.
Technical specifications
- High-throughput cloning and transformation: Utilizes established systems for efficient plant transformation.
- Transient expression assays: Employs Nicotiana benthamiana for rapid testing of promoter activity.
- SNP analysis: Screens natural diversity or employs synthetic promoter design using techniques such as random mutagenesis and DNA synthesis.
- Quantitative data output: Provides quantitative measurements of promoter activity through advanced assay techniques.
Technology readiness level
The technology is currently at TRL 4, indicating that it has been validated in a laboratory environment. Future validation plans involve in silico analyses and further rounds of design-build-test-learn cycles to refine promoter designs according to specific research needs.
About University of East Anglia
The University of East Anglia (UEA) is a comprehensive public research university in Norwich, United Kingdom. The campus sits within a major research park alongside an NHS teaching hospital, enabling co‑located laboratories, clinical access, and regulated trials. Industry engagement includes placements, contract research, consultancy, and fast‑track agreements delivered through a central research and innovation office. Research is funded by UK Research and Innovation and its councils, the National Institute for Health and Care Research, and major charitable foundations. A dedicated technology transfer office manages IP, licensing, and spinouts, with on‑campus innovation space for partners.
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