High throughput promoter variant screening in protoplasts using dual-luciferase system

Overview

Solis Agrosciences presents an innovative solution for high throughput screening of promoter variants in protoplasts using a dual-luciferase reporter system. This approach allows for the rapid assessment of single nucleotide polymorphisms (SNPs) and other mutations to determine their effects on gene expression. By leveraging protoplasts as a plant-based system, this technology provides meaningful data quickly and efficiently, making it possible to identify mutations that enhance expression and uncover regulatory elements of interest.

Technical specifications

Key features:

• Utilizes a dual-luciferase reporter system for precise quantification of promoter activity.
• Capable of screening large libraries of mutant promoters in a 96-well plate format.
• Supports high throughput capacity, handling up to 12 96-well plates per week.
• Employs robust biological replication, ensuring definitive findings.
• Provides comprehensive data on yield, viability, and transfection rates using cell imaging.
• Suitable for use in corn and tomato protoplasts, with potential applications in other plant systems.

Technology readiness level

The technology is currently at TRL 5, having been validated with preliminary data in corn protoplasts. The next steps involve further validation and deployment in additional plant systems, with the goal of identifying and exploiting beneficial mutations for agricultural advancements.