HTS methodologies for studying immune-fibroblast crosstalk in wound healing
This innovative solution leverages high-throughput screening (HTS) methodologies to study the interaction between innate immune cells and fibroblasts in wound healing. It enables the analysis of scratch assays, ECM formation, and immune signaling using accessible systems.
Overview
This state-of-the-art solution employs high-throughput screening (HTS) methodologies to explore the interaction between innate immune cells and fibroblasts in wound healing. By utilizing a co-culture system of human model macrophages, dendritic cells, and fibroblasts, the solution facilitates a versatile analysis of wound healing processes. Key outputs include scratch assays, ECM formation, and immune signaling, all measurable through accessible systems like the Incucyte Live Cell Analysis system and fluorescent reporter systems.
Technical specifications
- Co-culture system: Involves model macrophages, dendritic cells, and fibroblasts co-cultured on 96-well plates.
- Analysis methods: Includes in vitro scratch healing models, ECM formulation, and secretion of wound healing factors such as GDF15 and VEGF.
- Fluorescent labeling: Utilizes genetically engineered fibroblasts and immune cells expressing GFP, RFP, and YFP to track ECM and immune factor secretion.
- Additional assays: Capable of performing ELISA and bead array assays to enhance data reliability.
- Infectious simulation: Offers the option to incorporate bacteria to simulate infectious conditions for comprehensive analysis.
Technology readiness level
The technology is currently at TRL 2, reflecting its validation in laboratory settings with plans for further in vivo validation using mouse models.
About Drexel University
Drexel University is a comprehensive private research university in Philadelphia, recognized for an urban, industry‑embedded model anchored by a longstanding cooperative education program. Year‑round co‑ops create a ready talent pipeline and align sponsored research with real‑world needs. The campus sits within an innovation district with co‑located labs and incubators, enabling companies to collaborate on prototyping with faculty. Through the university’s medical college and clinical partners, industry teams can access clinical expertise and translational pathways. Research is supported by competitive federal funding from agencies such as NIH, NSF, DOE, and DoD. A dedicated technology transfer office manages IP, licensing, corporate research agreements, and startup formation.
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