Impetus ag: bt trait rescue for resistant FAW control using bispecific VHH antibodies

Overview

Impetus Ag has developed a novel approach to combat resistant Fall Armyworm (FAW) by utilizing bispecific VHH antibodies (BsVHH). These antibodies 'tether' Bt toxins to novel mid-gut receptors, effectively restoring control over resistant strains. This innovative solution is applicable in both topical applications, where BsVHHs are expressed in Bt strains for spray-on use, and in-plant applications, where they are co-expressed with Bt transgenes to enhance durability. The technology is modular, potentially extending to other pests such as Corn Earworm (CEW) and Diamondback Moth (DBM).

Technical specifications

• BsVHH Design: Utilizes synthetic VHH libraries and AI-guided design for novel, specific binding sequences.
• Product Modalities: Two options - topical (spray-on) and in-plant (genetically integrated).
• Manufacturing: Compatible with standard Bt fermentation; BsVHH expression does not affect high toxin yields.
• Potency Improvement: Demonstrated 50% increase in potency against resistant FAW compared to Cry toxins alone.
• Platform Capabilities: Modular and adaptable for additional pests.

Technology readiness level

The technology is currently at TRL 4, with ongoing validation processes. Phase 1 focuses on optimizing BsVHH lead candidates, quantifying expression, and running GLP-like diet assays. Phase 2 will expand validation to other pests, refine manufacturability, and conduct greenhouse tests on conventional and traited corn. The aim is to reach TRL 5 with further development.