In-field rapid DNA/RNA detection in plants using aptasensor technology

Overview

This project develops a novel, portable aptasensor combined with electrochemical potentiostat technology for rapid, in-field detection of DNA and RNA genetic features in plant material. The solution specifically targets the CP4 EPSPS gene in GMO crops, delivering high sensitivity and specificity within 30 minutes without the need for DNA/RNA purification. Designed for diverse environments including greenhouses and warehouses, the technology addresses the growing market and regulatory demand for on-site, point-of-care diagnostics in agriculture.

The aptasensor is engineered to accommodate larger genetic inserts and single nucleotide polymorphisms, with potential for quantitative analysis and simultaneous detection of multiple targets. By eliminating amplification steps and purification requirements, the platform offers a scalable, cost-effective solution for rapid genetic quality assessment of leaf and seed material.

Technical specifications

Key features:

• Portable, field-deployable aptasensor design for direct plant material testing
• Electrochemical potentiostat technology providing cost-effective, chip-level instrumentation
• Amplification-free detection workflow eliminating the need for DNA/RNA purification
• Aptamer-based recognition enabling detection of larger genetic inserts and single nucleotide polymorphisms
• Disposable electrode integration for streamlined sampling and testing within a single device
• Potential for multiplexed detection of multiple DNA targets simultaneously
• 30-minute time-to-result for rapid genetic quality assessment
• Compatibility with greenhouse, warehouse, and field agricultural environments

Development approach:

• Target-specific aptasensor design and integration onto disposable electrodes
• Sensitivity, specificity, and limit of detection characterization with target plant DNA
• Single-device integration combining plant material sampling and testing
• System expansion for simultaneous multi-target detection
• Field testing in real-world agricultural settings
• Design refinement for user-friendliness, environmental sustainability, and regulatory compliance

Technology readiness level

The project is currently in the research and development phase, leveraging established expertise in electrochemical potentiostat technology previously commercialized at the chip level and validated with aptasensors in medical diagnostic applications. The collaboration with a point-of-care sensing expert brings substantial experience in designing versatile, deployable molecular-based testing platforms.

The planned six-phase validation pathway progresses from aptasensor design and laboratory characterization through field testing in agricultural settings, culminating in commercialization strategy development and regulatory approval navigation. This staged approach ensures systematic validation of sensitivity, specificity, real-world performance, and user requirements before market deployment.