In vitro spatial proteomics platform for parkinson's disease research
A novel in vitro platform using LUHMES cells and high-throughput imaging to study cerebrospinal fluid-induced proteomic changes in Parkinson's Disease, aiming to identify new molecular targets and enhance drug screening.
Overview
This innovative research platform focuses on understanding the proteomic changes induced by cerebrospinal fluid (CSF) from Parkinson's Disease (PD) patients in dopaminergic neurons. Utilizing LUHMES cells, a human dopaminergic neuron model, and integrating high-throughput antibody-based imaging from the Human Protein Atlas, the platform aims to map CSF-induced proteomic perturbations. This approach seeks to uncover novel molecular targets associated with PD pathology, offering insights into synaptic dysfunction, protein misfolding, inflammation, and apoptosis, which are key neurodegenerative hallmarks.
Technical specifications
- Model System: LUHMES cells differentiated into dopaminergic neurons, providing a robust human model.
- Proteomic Analysis: High-throughput, single-cell spatial proteomics integrated with antibody-based imaging.
- Protein Coverage: Utilizes the Human Protein Atlas with approximately 28,000 antibodies covering 87% of the proteome.
- Machine Learning: Classification of protein localization and abundance to improve reproducibility in drug screening.
- Target Proteins: Focus on ~400 key proteins linked to neuronal health and PD pathology.
Technology readiness level
This platform is currently at Technology Readiness Level 3, indicating it is in the active research and proof of concept stage. Future validation includes developing a scalable in vitro assay for PD research and therapeutic development.
About KTH Royal Institute of Technology
KTH Royal Institute of Technology is Sweden’s flagship technical university in Stockholm, a comprehensive STEM-focused institution spanning multiple campuses and applied research environments. Industry engages through co-located labs, urban testbeds, prototyping workshops, and pilot-scale facilities designed for joint development. An embedded presence in Kista Science City connects partners to a dense ICT cluster, while structured industrial PhD pathways and flexible research agreements enable long-term collaboration. Research is supported by competitive funding from Swedish national agencies and European programs. A dedicated technology transfer office, incubator support, and seed financing streamline IP protection, licensing, and venture creation.
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