Investigating TAS2R activation in stomach due to VMS tablets

Overview

This project investigates whether multivitamin and mineral supplements (VMS) tablets cause nausea through the activation of bitter taste receptors (TAS2Rs) in the stomach. Unlike natural food sources, VMS tablets contain high concentrations of vitamins and minerals, which may activate TAS2Rs, leading to increased gastric acid secretion and delayed gastric emptying. The research aims to confirm TAS2R activation by VMS and explore strategies to reduce these adverse effects.

Technical specifications

Research Hypothesis: TAS2Rs, present in the stomach, are activated by high concentrations of vitamins in VMS tablets, causing nausea.

• Methodology:

  • Screening of all 26 TAS2Rs for activation by VMS using high-throughput calcium mobilization assays.
  • Analysis of responsive TAS2Rs for threshold and EC50 concentrations.
  • Testing of human stomach tissue samples for TAS2R gene expression using quantitative digital-droplet RT-PCR assays.

• Potential Solutions:

  • Application of known bitter blockers.
  • Identification of new bitter blockers or reformulation of VMS.

Technology readiness level

This research is at TRL 3, indicating experimental proof of concept. The team is equipped with plasmids, suitable cell lines, and high-throughput assays to validate the hypothesis and explore mitigation strategies.