LC-MS/MS method development and validation for Mycotoxin quantification
Fraunhofer IME offers specialized method development and validation services for quantifying Mycotoxins in grain extracts using advanced LC-MS/MS techniques. This service ensures accurate, precise, and reliable mycotoxin detection, crucial for food safety and quality control.
Overview
Fraunhofer IME provides method development and validation services for the quantification of Mycotoxins in grain extracts using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). This service is designed to deliver precise, accurate, and reliable detection of Mycotoxins, which are harmful compounds affecting food safety. The service includes method development with a focus on separation and detection using state-of-the-art equipment, ensuring high standards of accuracy and specificity.
Technical specifications
- Methodology: Utilizes reversed-phase (U)HPLC-column with online UV-detection and tandem mass spectrometry.
- Equipment: Employs Ultimate3000 for HPLC and TSQ QuantumUltra for mass spectrometry.
- Detection Mode: Single Reaction Monitoring (SRM) with quantifier and qualifier ions.
- Validation: Follows ICH guidelines, addressing accuracy, precision, specificity, detection limit, quantification limit, linearity, and range.
- Proven Expertise: Builds on Fraunhofer IME's experience with analytical methods and tech transfers.
Technology readiness level
The method is currently at a TRL of 3, indicating development and validation in a laboratory environment. It is poised for further integration and validation with reference standards and real samples from industry partners, with the potential for tech-transfer to industry applications.
About Fraunhofer Institute
Fraunhofer is a large, multi-site applied research organization based in Germany, operating a nationwide network of institutes and research units with a strong industry-facing mission. Its contract-research model aligns work to real manufacturing and deployment needs, with pilot lines, test labs, and demonstration facilities for validation and scale-up. Many institutes are integrated with nearby universities and regional industry clusters, enabling joint appointments, shared infrastructure, and fast talent pipelines. Research is supported by competitive European programs alongside German federal and state funding, complemented by extensive contract revenue from private-sector collaborations. A dedicated technology transfer function manages IP, licensing, and startup formation.
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