Lignin nanoparticle delivery system for oligonucleotides

Overview

This solution involves the development of novel lignin-derived nanoparticles intended for the delivery of oligonucleotides to human neuron cells. By harnessing the natural amphiphilic properties of lignin, which include both hydrophobic and hydrophilic functional groups, these nanoparticles are designed to efficiently encapsulate and transport oligonucleotides. Such properties are expected to facilitate the crossing of cellular membranes, making this method particularly promising for applications targeting the blood-brain barrier (BBB), which is notoriously challenging for drug delivery.

Technical specifications

Key features:

• Utilizes lignin's amphiphilic nature, with aromatic rings and polar functional groups, to enhance encapsulation and delivery.
• Employs various types of lignin - guaiacyl, syringyl, and catechyl - to tailor the nanoparticle properties by varying hydroxyl and methoxyl group content.
• Size of nanoparticles can be controlled between 20-300 nm to optimize delivery efficiency.
• Uses solvent exchange method for encapsulation, enhancing permeability by increasing hydroxyl groups via demethylation.
• Tested delivery through the BBB using the hCMEC/D3 cell line, with siRNA fluorescently labeled for tracking.

Technology readiness level

This technology is currently at a Technology Readiness Level (TRL) of 2, indicating that the concept has been formulated and initial experimental proof of concept is underway. Future validation plans include further in vitro testing to assess delivery efficacy and pharmacokinetics.