Lipid oxidation measurement in animal proteins using TBARS and GC-MS
A novel approach for measuring lipid oxidation in dry animal proteins using the TBARS assay and GC-MS technology, focusing on quantifying hexanal and malondialdehyde (MDA) for efficient routine monitoring.
Overview
This research presents a dual-method approach to measure lipid oxidation in dry animal proteins. By utilizing gas chromatography-mass spectrometry (GC-MS) for detecting hexanal and the TBARS assay for measuring malondialdehyde (MDA), this method offers both precision and convenience. Hexanal serves as a marker for lipid oxidation, while MDA analysis through the TBARS assay provides a rapid and practical alternative for routine monitoring.
Technical specifications
- GC-MS Analysis: Offers detailed quantification of hexanal, a secondary product of lipid oxidation, albeit with a complex and time-consuming process.
- TBARS Assay: Provides a simpler, faster method to measure malondialdehyde (MDA), another byproduct of lipid peroxidation, making it suitable for routine applications.
- Protocol Readiness: The TBARS method is ready for implementation, requiring only equipment acquisition and technician training to begin.
Technology readiness level
The technology is at TRL 6, indicating that it has been demonstrated in a relevant environment and is ready for further validation through technician training and equipment setup.
About San Jose State University
San José State University is a comprehensive public R2 research university serving a large, diverse community in the heart of Silicon Valley. Industry engagement is built in: the Office of Innovation & Corporate Partnerships is a single front door for companies, and the SJSU Research Foundation supports contracting and compliance for sponsored projects. The College of Engineering’s co‑op program and industry advisory connections link corporate R&D to workforce‑ready talent. New facilities, including the Interdisciplinary Science Building, provide modern labs and collaboration spaces downtown. Research is supported by competitive federal funding from agencies such as NSF, NIH, and NASA.
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