Low-pressure subcritical CO2 isolation of non-cell-associated nucleic acid
Ousia Labs proposes using subcritical CO2 extraction to isolate non-cell-associated nucleic acid from biological samples. This gentle, non-destructive method preserves organism viability while enabling selective compound extraction. The approach leverages proprietary CO2 systems already adopted by universities for streamlined laboratory workflows.
Overview
Ousia Labs is developing a low-pressure subcritical CO2 extraction method specifically designed for isolating non-cell-associated nucleic acid from biological samples. The technique offers a gentle, non-destructive alternative to traditional solvent-based isolation methods, preserving the structural integrity and viability of living organisms throughout the extraction process. By using subcritical CO2 as a solvent, the approach can operate in a solvent-free or reduced-solvent mode, minimizing chemical impact on delicate biological specimens. The method is designed to deliver a streamlined workflow suitable for diverse laboratory environments, from academic research settings to professional biotech facilities.
Technical specifications
- Selective extraction: Subcritical CO2 selectively isolates target nucleic acid compounds while maintaining biological sample viability
- Gentle processing: Low-pressure, non-destructive conditions preserve structural integrity of living organisms
- Solvent-free or reduced-solvent operation: Minimizes chemical contamination and biological sample disruption
- Controllable parameters: Systematic optimization of time, temperature, and micron size for maximum selectivity and yield
- Analytical validation: Integrated assessment of quantity, purity, and integrity of isolated nucleic acid
- Proprietary equipment: Builds on Ousia Labs' existing CO2 extraction platform already deployed at universities including Bakersfield and Cornell
- Practical workflow design: Equipment and procedure package engineered for easy implementation across diverse laboratory settings
Technology readiness level
The technology is at an advanced validation stage. The underlying CO2 extraction platform has already been adopted and utilized by academic institutions including Bakersfield and Cornell Universities, providing real-world validation of the core equipment. Test results from isolating compounds sourced from diverse biomaterials have further confirmed the method's viability. Remaining development focuses on optimizing control parameters specifically for nucleic acid isolation, refining those parameters based on analytical testing outcomes, and confirming reproducibility through repeated analytical testing. The final deliverable includes a complete equipment and procedure package designed for practical deployment in research laboratories.
About Ousia Labs
Ousia Labs specializes in developing CO2 extraction technology, making professional-grade botanical oil extraction accessible through its flagship home appliance, the Ousia Fountain. By utilizing a compact, user-friendly, and safe extraction method that functions on kitchen countertops, the company enables individuals to produce pure, chemical-free essential oils from herbs, spices, and flowers for use in culinary applications, fragrance creation, aromatherapy, and cosmetics. The system is designed to provide high-quality output while eliminating the need for complex, industrial-scale equipment previously required for such processes.
Beyond consumer-facing appliances, Ousia Labs serves the professional, research, and laboratory sectors by offering lab-grade extraction systems designed for consistency and precision. These higher-volume solutions, such as the 3.2LSFE model, provide advanced features like integrated flash chromatography and precise flow control to support businesses and universities. The company further augments its technology through the Ousia AI application, which assists users by identifying botanical compounds and recommending optimal extraction parameters based on desired outcomes, thereby helping customers maintain control over their wellness and ingredient quality.
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