Mass spectrometry-based screening for aromatase-driven androgen to estrogen conversion
A novel mass spectrometry approach to study CYP19 aromatase's enzymatic conversion of androgens to estrogens, offering insights into estrogen biosynthesis and potential therapeutic strategies for estrogen-dependent diseases.
Overview
This innovative research utilizes mass spectrometry to analyze the enzymatic activity of recombinant CYP19 (aromatase) in converting androgens such as testosterone and androstenedione into estrogens, specifically estradiol and estrone. By focusing on the catalytic properties and regulatory mechanisms of CYP19, this study aims to provide valuable insights into estrogen biosynthesis, which is crucial for understanding estrogen-related physiological processes and developing therapeutic strategies for conditions that are dependent on estrogen levels.
Technical specifications
Core Technology:
- Utilizes mass spectrometry-based screening to quantify conversion efficiency and product specificity of CYP19.
- Builds on previously successful methodologies applied to SARS-CoV-2 protease studies.
- Involves the expression and purification of recombinant CYP19 for in vitro analysis.
Key Features:
- High-throughput mass spectrometry (HTMS) for precise quantification of enzymatic activity.
- Analysis of androgen-to-estrogen conversion rates under controlled conditions.
- Insights into fundamental mechanisms of aromatase function and estrogen production.
Technology readiness level
This research is currently at Technology Readiness Level 3, indicating that analytical and experimental studies are underway to validate the technology's feasibility and efficacy in controlled environments.
About University of Michigan
The University of Michigan is a comprehensive public research university based in Ann Arbor with additional campuses in Dearborn and Flint, known for a broad, interdisciplinary research enterprise and a major academic health system. Industry partners engage through co-located facilities, including a large north campus research complex with shared labs and incubator space, plus on-campus testbeds for rapid prototyping and validation. Proximity to Detroit’s mobility and manufacturing base, plus dedicated business engagement teams, streamlines sponsored research and access to talent. Research is supported by competitive federal funding from agencies such as NIH, NSF, DOE, and DoD, alongside state and industry sponsorship. A centralized tech transfer office manages IP, licensing, and startup support, with corporate memberships and flexible agreements.
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