Molecular detection of tobamoviruses in seeds using luminex xtag
A novel molecular detection method using Luminex xTAG for assessing tobamovirus viability in seeds by testing viral RNA integrity. Proven effective in detecting cucumber green mottle mosaic virus and pepino mosaic virus, with potential for broad virus detection applications.
Overview
This innovative solution employs the Luminex xTAG system for the molecular detection of tobamoviruses in seeds. The method focuses on assessing the integrity of viral RNA to determine virus viability. By detecting multiple longer RNA stretches, it provides a reliable measure of virus infectiousness, crucial for seed disinfection processes.
Technical specifications
The process involves RT-PCR based amplification of virus-specific sequences, followed by linear amplification and detection of PCR products. This method has been validated with cucumber green mottle mosaic virus (CGMMV) and pepino mosaic virus (PepMV), demonstrating its versatility and effectiveness. The protocol is adaptable to various viruses by selecting appropriate RT-PCR primers and TSPE primers to cover significant portions of the viral genome.
Technology readiness level
Currently at TRL 5, the technology has undergone proof-of-principle validations and is being further developed for broader applications. Future validation plans include testing additional tobamoviruses and refining the primer combinations to enhance detection accuracy and reliability.
About Wageningen University and Research
Wageningen University & Research is a life sciences–focused public university combined with mission-driven research institutes, integrating fundamental, applied, and field-based R&D at scale. On Wageningen Campus, companies co-locate with WUR teams and use shared pilot plants, advanced analytical labs, and controlled‑environment facilities for rapid prototyping. Being in the Food Valley cluster provides tight links to global corporates, SMEs, and startups through living labs and a strong regional talent pipeline. Research is backed by competitive funding from the Dutch Research Council, European Union programs, and national ministries, alongside significant contract research for industry. A dedicated technology transfer office and campus incubators streamline IP, licensing, and spin‑out formation for corporate partners and entrepreneurs.
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