Mrna-targeted rt-qpcr for viable salmonella detection in food
This innovative RT-qPCR method selectively detects viable Salmonella cells in food samples by targeting mRNA biomarkers, enhancing sensitivity and specificity over traditional DNA-based methods.
Overview
This cutting-edge solution utilizes mRNA-targeted real-time reverse transcription PCR (RT-qPCR) to detect viable Salmonella cells in complex food samples with high sensitivity and specificity. By focusing on mRNA biomarkers, which degrade rapidly after cell death, this method effectively distinguishes between live and dead cells, minimizing false positives and providing a more accurate assessment of microbial food safety risks compared to conventional DNA-based detection methods.
Technical specifications
- Key features:
- Targets specific mRNA transcripts such as invA and hilA expressed in metabolically active Salmonella cells.
- Integrates optimized RNA extraction protocols to ensure high sensitivity and specificity.
- Includes an enrichment step to increase bacterial concentration, capable of detecting nearly 1 CFU/kg or per L.
- Validated on diverse food matrices like cocoa and chocolate, ensuring robust performance.
- Cost-effective with a target cost of 50 Euros per test.
Technology readiness level
Currently at Technology Readiness Level 2, this solution is in the validation phase, with ongoing testing across various food matrices to ensure its efficacy and reliability.
About University of Florida
The University of Florida is a comprehensive public research university with a broad academic and research portfolio and a statewide presence. Industry collaborates through co-located labs and shared core facilities and through an integrated academic health system that accelerates clinical translation. A statewide extension network and multiple research and education sites connect companies with field-scale testing and rapid deployment, while incubators and an adjacent innovation district provide pathways from lab to market. Research is supported by competitive funding from major federal agencies such as NIH, NSF, USDA, and DOE. A dedicated technology transfer office supports IP, licensing, and startup formation.
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