Multi-enzyme cascade system for controlling candida albicans biofilm
A novel multi-enzyme cascade system utilizes polysaccharide-degrading enzymes and oxidoreductases to disrupt Candida albicans biofilm by degrading the matrix and killing embedded cells with H2O2. This synergistic approach enhances antibiofilm activity.
Overview
This innovative solution involves a multi-enzyme cascade system designed to control Candida albicans biofilm formation. It integrates polysaccharide-degrading enzymes and oxidoreductases, which collaboratively degrade the biofilm matrix and produce hydrogen peroxide (H2O2) to kill embedded fungal cells. This dual-action mechanism not only disrupts the biofilm structure but also reduces fungal cell viability with minimal side effects, offering a potent method to manage biofilm-related infections.
Technical specifications
The core of this technology is a two-step enzymatic process. First, polysaccharide-degrading enzymes such as glucanases break down the biofilm's matrix polysaccharides, primarily poly-β-glucose, into glucose. Subsequently, oxidoreductases catalyze the conversion of glucose into H2O2. This hydrogen peroxide acts as a biocide and an oxidizing agent, effectively detaching and eradicating the biofilm. The system's adaptability allows for the incorporation of various enzyme combinations to optimize antibiofilm efficacy.
Technology readiness level
This technology has reached TRL 6, indicating that it has been demonstrated in a relevant environment. Future validation efforts will focus on testing the antibiofilm effects of different enzyme combinations, studying the synergistic mechanisms, and enhancing the system through enzyme engineering and cross-linking methods.
About Qingdao Agricultural University
Qingdao Agricultural University is a public, multi‑campus university in Shandong serving over 30,000 students and anchored in agriculture with broad complementary fields. A province‑wide network of model bases, regional institutes, and field stations enables on‑farm validation, producer training, and scale‑up with enterprise partners. The Modern Agri‑tech Service Center in Jiaozhou and the Blue Valley campus place faculty near growers, processors, and the city’s marine innovation corridor. Research is backed by competitive national and provincial programs, including the National Natural Science Foundation of China and the Ministry of Science and Technology. A dedicated technology transfer office supports IP, licensing, and school–enterprise agreements to speed commercialization.
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