Multiplex digital PCR for same-day, cost-effective genotyping
This innovative digital PCR technology enables rapid, multiplex genotyping with same-day results. Utilizing an 8-color instrument and dual-color combination multiplexing, it efficiently determines multiple SNPs, offering scalability and simplicity in setup and analysis.
Overview
This solution leverages multiplex digital PCR technology to deliver rapid, cost-effective genotyping results within the same day. By employing a higher-order multiplexing strategy, it quantifies more genetic targets than traditional fluorescent detection channels allow. With an 8-color instrument using dual-color combination multiplexing, the system can determine 14 bi-allelic SNPs in a single reaction, providing significant scalability and efficiency. The setup is straightforward, with simple PCR workflows and non-complex data analysis manageable on a standard laptop.
Technical specifications
- Higher-order multiplexing: Allows for the detection of more targets than the number of fluorescent channels available.
- Scalability: Capable of typing 14 SNPs in 96 samples, 28 SNPs in 48 samples, or 56 SNPs in 24 samples in under 3 hours.
- Instrumentation: Utilizes an 8-color PCR instrument, scalable by increasing the number of instruments or using advanced setups.
- Design pipeline: Includes allele-specific primers, universal probes, and software for automated data analysis.
- Flexibility: Capable of direct PCR on non-extracted samples such as saliva or cheek swabs.
Technology readiness level
The technology is at TRL 5, indicating that validation in relevant environments is underway. The system is designed for easy scalability and straightforward technology transfer and training. Future validation aims to extend the multiplexing capability and optimize direct PCR conditions for various sample types.
About pxlence
pxlence is a Ghent University spin-off established in 2015 that specializes in the development and manufacturing of PCR-based assays and kits for targeted resequencing and nucleic acid quantification. The company offers three core product lines: a catalogue of nearly one million predesigned PCR assays for targeted resequencing of human exonic regions; universal Rainbow DNA detection probes designed for digital PCR and quantitative PCR to streamline multiplexing; and Human Sample ID kits used for DNA authentication and sample tracking. Their technology centers on a proprietary high-throughput primer design pipeline that allows for high-performance amplification and precise genetic targeting across various sample types, including genomic DNA, FFPE, and cfDNA.
These solutions are primarily utilized by clinical diagnostic laboratories and medical device companies to address critical needs in molecular diagnostics and precision medicine. By providing standardized, high-quality assays that integrate with NGS workflows and digital PCR platforms, pxlence helps researchers and diagnostic professionals improve accuracy, reduce costs, and simplify experimental design. The company has demonstrated the efficacy of its tools through peer-reviewed research and collaborative projects aimed at advancing cancer detection and genetic variant analysis, supporting the broader healthcare industry in enhancing diagnostic reliability and throughput.
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