Multiplex lateral flow assay for salmonella spp. serotyping
A multiplex lateral flow assay using monoclonal antibodies and magnetic nanoparticles to improve detection and enable Salmonella spp. serotyping via O and H (flagellar) antigens. The approach is intended to increase specificity and sensitivity, reduce false positives and negatives, and deliver faster, more accurate results than standard culture or PCR workflows. Double-blind testing is planned to validate performance, with a 12–24 month path toward a market-ready diagnostic product.
Overview
The multiplex lateral flow assay is a diagnostic tool designed to enhance serotyping of Salmonella spp. By targeting O and H antigens located on the flagellum, the assay provides a more reliable and efficient method for identifying various Salmonella strains. Incorporating monoclonal antibodies and magnetic nanoparticles is intended to improve the detection limit, supporting more accurate and rapid diagnosis compared to traditional methods.
Technical specifications
- Uses O and H antigens for serotyping through a lateral flow assay mechanism
- Incorporates monoclonal antibodies to improve specificity and sensitivity
- Uses magnetic nanoparticles to enhance the detection limit for more precise results
- Intended to reduce false positives and negatives, with performance positioned to outperform PCR and standard culture tests
- Planned double-blind testing to validate performance
Technology readiness level
Currently at Technology Readiness Level 4, the assay has been developed and is undergoing further testing. With a projected timeline of 12–24 months, the goal is to create a market-ready diagnostic product following comprehensive validation processes.
About University of Wisconsin, Milwaukee
University of Wisconsin–Milwaukee is a comprehensive public research university in the state’s largest metro, pairing broad academic breadth with an applied, urban mission. Industry engages through an innovation campus and research park that co-locate university labs with startups and corporate teams, plus shared-use core facilities. Its location puts partners near a major regional medical center and within a leading freshwater and advanced manufacturing cluster, enabling pilots, internships, and joint development. Research is supported by competitive federal funding from agencies such as NSF, NIH, and DOE. A dedicated technology transfer office and affiliated foundation manage IP, licensing, corporate agreements, and seed funding to accelerate commercialization.
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