Multiplexed SERS-VFIA for rapid vitamin detection in urine
A novel platform using multiplexed SERS and VFIA integrated with machine learning for rapid, cost-effective vitamin detection in urine. This solution enhances diagnostic efficiency and accessibility by enabling sensitive, multiplexed vitamin analysis at point-of-care.
Overview
The multiplexed Surface-Enhanced Raman Spectroscopy (SERS) combined with Vertical Flow Immunoassay (VFIA) presents a groundbreaking method for detecting vitamins in urine. This platform is designed to offer rapid, cost-effective, and user-friendly testing capabilities suitable for point-of-care applications. By integrating SERS with VFIA and machine learning algorithms, this solution enables sensitive and specific detection of multiple vitamins simultaneously, improving diagnostic efficiency and accessibility. This approach addresses the limitations of conventional assays, which are often time-consuming and require complex instrumentation.
Technical specifications
- SERS Technology: Provides high sensitivity and specificity necessary for detecting low-abundance analytes.
- VFIA Platform: Offers rapid, low-cost testing suitable for clinical and point-of-care settings.
- Machine Learning Integration: Enhances the identification and quantification of spectral features for accurate vitamin detection.
- Cost-Effective Development: The Raman spectrometer for point-of-care applications can be developed for less than $6500.
- Current Validation: Successful detection of vitamin K3 and integration with immunoassays for protein biomarkers.
Technology readiness level
The technology is currently at TRL 3, having demonstrated proof-of-concept for detecting vitamins and integrating SERS with immunoassays. Further development will focus on characterizing SERS spectra for vitamins D, B6, B2, and K in urine and the construction of a cost-effective Raman spectrometer.
About University of Tennessee, Knoxville
UT offers a partnership model that reduces risk, accelerates time-to-value, and supports long-term growth strategies for industry—providing an attractive platform for companies seeking to formalize collaboration and establish a long-term presence in the East Tennessee region. By aligning institutional investment with regional and national priorities, UT provides a stable and effective platform for industry collaboration, technology deployment, and sustained economic impact.
delivered to your inbox.