Murine modeling of hydration and transport using stable isotopes
A cutting-edge murine model utilizing stable isotopes to quantify the transport and bioaccumulation of water and amino acids, offering precise insights into hydration and metabolic processes.
Overview
This innovative research focuses on developing a murine model to study hydration and transport processes using stable isotopes. By co-administering oral rehydration solutions with isotopically labeled biomolecules, this approach enables precise quantification of water and amino acid transport from the gut to host tissues. This model can significantly enhance our understanding of hydration mechanisms, with potential applications extending to human studies.
Technical specifications
- Utilizes stable isotopes such as deuterated water (D2O) and isotopically labeled amino acids to investigate transport and metabolism.
- Integrates advanced techniques like RNAseq and RT-qPCR to assess transporter expression in response to isotopic labeling.
- Employs established murine models (C57BL/6 mice) for experimental validation, ensuring robust and reproducible results.
- Dose-response experiments to determine the half-life of labeled substrates in circulation, followed by tissue analysis using LC-MS/MS.
Technology readiness level
This technology is at TRL 9, indicating it has been validated in an operational environment and is ready for commercial deployment and application.
About Montana State University
Montana State University's substantial research enterprise growth has been accompanied by focused investments in innovation and research translation, creating new opportunities for companies to engage with MSU research and expertise. A particular strength is MSU’s network of specialized centers, institutes, and industry-accessible core facilities, which bring together multidisciplinary expertise, advanced research infrastructure, testing capabilities, and established pathways for working with industry partners. MSU is one of a select group of universities participating in the NSF's Accelerating Research Translation (ART) program, which is building the capacity and infrastructure needed to translate more research discoveries into solutions with real-world impact. The growing MSU Innovation Campus and expanding incubator infrastructure provide additional opportunities for university-industry interaction, startup development, and technology-focused companies. These efforts are complemented by MSU’s Technology Transfer Office, which works with researchers and companies to protect and license MSU inventions and facilitate industry-sponsored research collaborations.
delivered to your inbox.