Natural product discovery and biosynthetic gene elucidation
A cutting-edge methodology for discovering, purifying, and elucidating the function of natural products through biochemical assays and genome sequencing. Offers solutions for compound overproduction using synthetic biology approaches.
Overview
This innovative approach focuses on the discovery, purification, and functional elucidation of natural products. By leveraging biochemical activity assays against target cells and genome sequencing, we identify and overproduce compounds like pulvomycin and siamycin I. Our method integrates synthetic biology to enhance compound production, providing significant potential for pharmaceutical applications.
Technical specifications
Key features:
- Biochemical identification of natural products through activity assays and genome sequencing
- Successful detection, purification, and mechanism elucidation of compounds such as pulvomycin and siamycin I
- Use of synthetic genes and chassis strains for high-level compound production
- Structural determination of natural products via mass spectrometry and NMR
- Genome sequencing with nanopore or pacbio for identifying PKS and NRP synthases
- Activity-guided fractionation and purification to homogeneity
Technology readiness level
This methodology has achieved a Technology Readiness Level of 5, indicating that it has been validated in a relevant environment. Future validation plans include activity assays on crude extracts and advanced potency measurements against resistant strains, ensuring readiness for more complex pathogen challenges.
About University of Toronto
The University of Toronto is a comprehensive public research university with three campuses in the Toronto region and a globally scaled research enterprise. Its downtown footprint is embedded within a major academic health network and an adjacent innovation district, enabling co-located labs, clinical trials, and rapid testing with end users. Companies connect through co-op and long-duration internships, sponsored research, and access to shared core facilities and prototyping resources. Research is supported by Canada’s Tri‑Agency (NSERC, CIHR, SSHRC) and the Canada Foundation for Innovation, alongside provincial programs and industry partnerships. A dedicated technology transfer office provides IP management, licensing, and startup support through a coordinated entrepreneurship network.
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